Regulation of ciliary motility by membrane potential in Paramecium: A role for cyclic AMP
Identifieur interne : 00E826 ( Main/Exploration ); précédent : 00E825; suivant : 00E827Regulation of ciliary motility by membrane potential in Paramecium: A role for cyclic AMP
Auteurs : Nancy M. Bonini [États-Unis] ; Michael C. Gustin [États-Unis] ; David L. Nelson [États-Unis]Source :
- Cell Motility and the Cytoskeleton [ 0886-1544 ] ; 1986.
English descriptors
- Teeft :
- Adenylate, Adenylate cyclase, Analog, Biol, Bonini, Brehm, Camp action, Camp level, Camp levels, Cation, Cell biol, Cell motil, Cells depolarized, Cholera toxin, Ciliary, Ciliary activity, Ciliary motility, Ciliary motion, Cilium, Continuous ciliary reversal, Control cells, Control solution, Culture dish, Cyclase, Cyclic, Cyclic nucleotide analogs, Cyclic nucleotides, Demembranated models, Depolarization, Depolarized, Depolarized cells, Depolarizing, Divalent cations, Eckert, Egta, Electrophysiological, Extracellular, Extracellular ibmx, Extracellular medium, Febs lett, Final concentration, First hypothesis, Gustin, Hyperpolarization, Hyperpolarized, Hyperpolarizing, Hyperpolarizing solutions, Ibmx, Intracellular, Kaneko, Kung, Machemer, Mechanical stimulation, Membrane, Membrane hyperpolarization, Mgatp, Motility, Mutant, Naitoh, Nakaoka, Nucleotide, Paramecium, Paramecium caudatum, Paramecium tetraurelia, Perchloric acid, Permeant, Phosphodiesterase, Phosphodiesterase inhibitors, Physiol, Reversal, Satow, Schultz, Separate cell incubations, Speed measurements, Speed response, Sperm, Time course, Transmembrane, Various concentrations.
Abstract
The membrane potential of Paramecium controls the frequency and direction of the ciliary beat, thus determining the cell's swimming behavior. Stimuli that hyperpolarize the membrane potential increase the ciliary beat frequency and therefore increase forward swimming speed. We have observed that (1) drugs that elevate intracellular cyclic AMP increased swimming speed 2–3‐fold, (2) hyperpolarizing the membrane potential by manipulation of extracellular cations (e.g., K+) induced both a transient increase in, and a higher sustained level of cyclic AMP compared to the control, and (3) the swimming speed of detergent‐permeabilized cells in MgATP was stimulated 2‐fold by the addition of cyclic AMP. Our results suggest that the membrane potential can regulate intracellular cAMP in Paramecium and that control of swimming speed by membrane potential may in part be mediated by cAMP.
Url:
DOI: 10.1002/cm.970060303
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 001B18
- to stream Istex, to step Curation: 001A98
- to stream Istex, to step Checkpoint: 003612
- to stream Main, to step Merge: 00F114
- to stream Main, to step Curation: 00E826
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Regulation of ciliary motility by membrane potential in Paramecium: A role for cyclic AMP</title><author><name sortKey="Bonini, Nancy M" sort="Bonini, Nancy M" uniqKey="Bonini N" first="Nancy M." last="Bonini">Nancy M. Bonini</name></author><author><name sortKey="Gustin, Michael C" sort="Gustin, Michael C" uniqKey="Gustin M" first="Michael C." last="Gustin">Michael C. Gustin</name></author><author><name sortKey="Nelson, David L" sort="Nelson, David L" uniqKey="Nelson D" first="David L." last="Nelson">David L. Nelson</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:760BF36BFA7221D8426D057D45749D339F8B49F6</idno><date when="1986" year="1986">1986</date><idno type="doi">10.1002/cm.970060303</idno><idno type="url">https://api.istex.fr/ark:/67375/WNG-M5MMDDBW-N/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">001B18</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">001B18</idno><idno type="wicri:Area/Istex/Curation">001A98</idno><idno type="wicri:Area/Istex/Checkpoint">003612</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">003612</idno><idno type="wicri:doubleKey">0886-1544:1986:Bonini N:regulation:of:ciliary</idno><idno type="wicri:Area/Main/Merge">00F114</idno><idno type="wicri:Area/Main/Curation">00E826</idno><idno type="wicri:Area/Main/Exploration">00E826</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Regulation of ciliary motility by membrane potential in <hi rend="italic">Paramecium</hi>: A role for cyclic AMP</title><author><name sortKey="Bonini, Nancy M" sort="Bonini, Nancy M" uniqKey="Bonini N" first="Nancy M." last="Bonini">Nancy M. Bonini</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Wisconsin</region></placeName><wicri:cityArea>Neurosciences Training Program, University of Wisconsin‐Madison, Madison</wicri:cityArea></affiliation><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Wisconsin</region></placeName><wicri:cityArea>Correspondence address: Department of Biochemistry, University of Wisconsin‐Madison, 420 Henry Mall, Madison</wicri:cityArea></affiliation></author><author><name sortKey="Gustin, Michael C" sort="Gustin, Michael C" uniqKey="Gustin M" first="Michael C." last="Gustin">Michael C. Gustin</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Wisconsin</region></placeName><wicri:cityArea>Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin Madison, Madison</wicri:cityArea></affiliation></author><author><name sortKey="Nelson, David L" sort="Nelson, David L" uniqKey="Nelson D" first="David L." last="Nelson">David L. Nelson</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Wisconsin</region></placeName><wicri:cityArea>Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin Madison, Madison</wicri:cityArea></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Cell Motility and the Cytoskeleton</title><title level="j" type="alt">CELL MOTILITY AND THE CYTOSKELETON</title><idno type="ISSN">0886-1544</idno><idno type="eISSN">1097-0169</idno><imprint><biblScope unit="vol">6</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="256">256</biblScope><biblScope unit="page" to="272">272</biblScope><biblScope unit="page-count">17</biblScope><publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher><pubPlace>Hoboken</pubPlace><date type="published" when="1986">1986</date></imprint><idno type="ISSN">0886-1544</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0886-1544</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Adenylate</term><term>Adenylate cyclase</term><term>Analog</term><term>Biol</term><term>Bonini</term><term>Brehm</term><term>Camp action</term><term>Camp level</term><term>Camp levels</term><term>Cation</term><term>Cell biol</term><term>Cell motil</term><term>Cells depolarized</term><term>Cholera toxin</term><term>Ciliary</term><term>Ciliary activity</term><term>Ciliary motility</term><term>Ciliary motion</term><term>Cilium</term><term>Continuous ciliary reversal</term><term>Control cells</term><term>Control solution</term><term>Culture dish</term><term>Cyclase</term><term>Cyclic</term><term>Cyclic nucleotide analogs</term><term>Cyclic nucleotides</term><term>Demembranated models</term><term>Depolarization</term><term>Depolarized</term><term>Depolarized cells</term><term>Depolarizing</term><term>Divalent cations</term><term>Eckert</term><term>Egta</term><term>Electrophysiological</term><term>Extracellular</term><term>Extracellular ibmx</term><term>Extracellular medium</term><term>Febs lett</term><term>Final concentration</term><term>First hypothesis</term><term>Gustin</term><term>Hyperpolarization</term><term>Hyperpolarized</term><term>Hyperpolarizing</term><term>Hyperpolarizing solutions</term><term>Ibmx</term><term>Intracellular</term><term>Kaneko</term><term>Kung</term><term>Machemer</term><term>Mechanical stimulation</term><term>Membrane</term><term>Membrane hyperpolarization</term><term>Mgatp</term><term>Motility</term><term>Mutant</term><term>Naitoh</term><term>Nakaoka</term><term>Nucleotide</term><term>Paramecium</term><term>Paramecium caudatum</term><term>Paramecium tetraurelia</term><term>Perchloric acid</term><term>Permeant</term><term>Phosphodiesterase</term><term>Phosphodiesterase inhibitors</term><term>Physiol</term><term>Reversal</term><term>Satow</term><term>Schultz</term><term>Separate cell incubations</term><term>Speed measurements</term><term>Speed response</term><term>Sperm</term><term>Time course</term><term>Transmembrane</term><term>Various concentrations</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The membrane potential of Paramecium controls the frequency and direction of the ciliary beat, thus determining the cell's swimming behavior. Stimuli that hyperpolarize the membrane potential increase the ciliary beat frequency and therefore increase forward swimming speed. We have observed that (1) drugs that elevate intracellular cyclic AMP increased swimming speed 2–3‐fold, (2) hyperpolarizing the membrane potential by manipulation of extracellular cations (e.g., K+) induced both a transient increase in, and a higher sustained level of cyclic AMP compared to the control, and (3) the swimming speed of detergent‐permeabilized cells in MgATP was stimulated 2‐fold by the addition of cyclic AMP. Our results suggest that the membrane potential can regulate intracellular cAMP in Paramecium and that control of swimming speed by membrane potential may in part be mediated by cAMP.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Wisconsin</li></region></list><tree><country name="États-Unis"><region name="Wisconsin"><name sortKey="Bonini, Nancy M" sort="Bonini, Nancy M" uniqKey="Bonini N" first="Nancy M." last="Bonini">Nancy M. Bonini</name></region><name sortKey="Bonini, Nancy M" sort="Bonini, Nancy M" uniqKey="Bonini N" first="Nancy M." last="Bonini">Nancy M. Bonini</name><name sortKey="Gustin, Michael C" sort="Gustin, Michael C" uniqKey="Gustin M" first="Michael C." last="Gustin">Michael C. Gustin</name><name sortKey="Nelson, David L" sort="Nelson, David L" uniqKey="Nelson D" first="David L." last="Nelson">David L. Nelson</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Lorraine/explor/InforLorV4/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 00E826 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 00E826 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Lorraine
|area= InforLorV4
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:760BF36BFA7221D8426D057D45749D339F8B49F6
|texte= Regulation of ciliary motility by membrane potential in Paramecium: A role for cyclic AMP
}}
| This area was generated with Dilib version V0.6.33. |  |